If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. Privacy Policy. 3 Prepare gel a. We will not share your information for any other purposes. NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. Your feedback has been submitted. LÉ´4 S S6måã°­‰Jİ�ÏKvt™:3)«7ßKl¢©àËf5Øòÿ ““ Prepare buffers Add 50 mL of 20X NuPAGE™ MES or MOPS SDS Running Buffer to 950 mL of deionized water to prepare 1X SDS Running Buffer. We appreciate your feedback. 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting. For reduced samples, add 1 mL of NuPAGE™ Antioxidant to 400 mL 1X SDS Running Buffer. These products typically do not have pictures or detailed descriptions. Description NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer.  MES or MOPS () serve as the trailing ion in the running buffer. For Research Use Only. H‰Òw6PH.V0 C…âä. The NuPAGEfiLDS Sample Buffer is a 4X concentrated solution containing twice as much LDS as the 2X concentration of NovexfiTris-Glycine SDS or Tricine SDS Sample Buffer. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. Description NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. However, we are committed to improving your shopping experience. 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting. NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes. Description. Remove the comb, and rinse the gel wells three times using 1X Running Buffer. Not for use in diagnostic procedures. The unique WedgeWell design of the Bolt Bis-Tris Plus gel allows loading of up to 2X more sample volume than other precast gels. Please sign in to view account pricing and product availability. NuPAGE Transfer Buffer (20X) is used to transfer proteins from NuPAGE Bis-Tris and NuPAGE Tris-Acetate gels to membranes for western blotting. Fisher Scientific is always working to improve our content for you. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes. You can also view your contract price by searching for this item(s) on GSA Advantage. Please use the form below to provide feedback related to the content on this product. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes. It maintains the neutral pH environment established during electrophoresis. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. NuPAGE®Transfer Buffer (20X) 50 mL 50 mL NuPAGE®Antioxidant 1 mL -- Methanol* 100 mL 100 mL Deionized Water 849 mL 850 mL Total Volume 1,000 mL 1,000 mL *NuPAGE®Transfer Buffer with 10% methanol provides optimal transfer of a single gel in the blot module. Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting. 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting. NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. All contact information provided shall also be maintained in accordance with our b. endstream endobj 94 0 obj <>/ExtGState<>>>/Subtype/Form>>stream NuPAGE and Bolt gels are a good choice before downstream transfer and sensitive western blot analysis and any other technique where protein integrity is crucial. This product(s) resides on a Fisher Scientific GSA or VA contract.